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Hoefer iso-dalt apparatus
Iso Dalt Apparatus, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iso-dalt+apparatus/iso+dalt+apparatus/pmc03033794-218-8-10
Average 90 stars, based on 1 article reviews
iso-dalt apparatus - by Bioz Stars, 2026-10
90/100 stars

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Stripping Membranes:

Article Title: Growth-differentiation factor-8 (GDF-8) in the uterus: its identification and functional significance in the golden hamster
Article Snippet: After first dimensional separation, the gel strip was soaked sequentially in equilibrium buffer pH 6.8 made up of 6 M urea, 30%(w/v) glycerol, 2%(w/v) SDS, 0.05 M Tris-HCl, 2%(w/v) DTT for 30 min and then in 2% iodoacetamide for 30 min. Second dimension separation in 10% SDS gel (18 × 16 cm) was carried out in an ISO-DALT apparatus (Hoefer Scientific Instruments, Amersham Biosciences, San Francisco, CA, USA) operated at a constant current of 60 mA.

Article Title: Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation
Article Snippet: The 2-DE was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments) at room temperature under constant voltage 100 V till the dye front reached the bottom of the gel.

Article Title: Mass-spectrometrical analysis of proteins encoded on chromosome 21 in human fetal brain.
Article Snippet: Overexpression of chromosome 21 genes is directly or indirectly responsible for the Down syndrome phenotype.. In order to analyse chromosome 21 gene products (Chr21Ps), we extracted proteins from fetal human brain cortex and applied an ultracentrifugal and chromatographic prefractionation principle followed by two-dimensional gel electrophoresis (2-DE) and mass-spectrometrical analysis using high-throughput automated MALDI-TOF=TOF.. Nine Chr21Ps were identified: pyridoxal kinase; superoxide dismutase [Cu=Zn] 1; carbonyl reductase 1; ES1 protein homolog, mitochondrial [Precursor]; cystathionine-beta-synthetase; T-complex protein 1, theta subunit; cystatin B; 6-phosphofructokinase; glycinamide ribonucleotide synthetase.

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells
Article Snippet: The second dimension electrophoresis was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments, San Francisco, USA).

Article Title: Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach
Article Snippet: The IPG strips were equilibrated to reduce the disulfide linkages through the addition of 10 ml of equilibrating solution containing isopropanol and 2.6% tributyl phosphine (Fluka) and then were gently rocked for 25 min. Second-dimension electrophoresis was performed using 9-16% gradient gels and the Iso-DALT apparatus (Hoefer Scientific Instruments, San Francisco, CA), and was then stopped when the tracking dye reached the anode end of the gels.

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
Article Snippet: The second dimension electrophoresis was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments, San Francisco, USA).

Article Title: Comparative proteomic analysis reveals differentially expressed proteins regulated by a potential tumor promoter, BRE, in human esophageal carcinoma cells.
Article Snippet: Esophageal tumorigenesis is a complex and cascading process, involving the interaction of many genes and proteins.. In this study, we have used the comparative proteomic approach to identify tumor-associated proteins and explore the carcinogenic mechanisms.. Two-dimensional electrophoresis (2-DE) and MALDI-TOF MS analysis of esophageal carcinoma and control cells revealed 10 proteins that were upregulated.

Article Title: Cyclin I and p53 are differentially expressed during the terminal differentiation of the postnatal mouse heart.
Article Snippet: In this study, we have used Ki-67 and MF20 mAb to determine how extensively cardiomyocytes proliferate in the postnatal mouse heart.. It was established that the cardiomyocytes divided rapidly in 2-day-old hearts.. However, at 13 days, the majority of cardiomyocytes had entered into terminal growth arrest and differentiation.

SDS-Gel:

Article Title: Growth-differentiation factor-8 (GDF-8) in the uterus: its identification and functional significance in the golden hamster
Article Snippet: After first dimensional separation, the gel strip was soaked sequentially in equilibrium buffer pH 6.8 made up of 6 M urea, 30%(w/v) glycerol, 2%(w/v) SDS, 0.05 M Tris-HCl, 2%(w/v) DTT for 30 min and then in 2% iodoacetamide for 30 min. Second dimension separation in 10% SDS gel (18 × 16 cm) was carried out in an ISO-DALT apparatus (Hoefer Scientific Instruments, Amersham Biosciences, San Francisco, CA, USA) operated at a constant current of 60 mA.

Article Title: Silencing BRE Expression in Human Umbilical Cord Perivascular (HUCPV) Progenitor Cells Accelerates Osteogenic and Chondrogenic Differentiation
Article Snippet: The 2-DE was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments) at room temperature under constant voltage 100 V till the dye front reached the bottom of the gel.

Article Title: Mass-spectrometrical analysis of proteins encoded on chromosome 21 in human fetal brain.
Article Snippet: Overexpression of chromosome 21 genes is directly or indirectly responsible for the Down syndrome phenotype.. In order to analyse chromosome 21 gene products (Chr21Ps), we extracted proteins from fetal human brain cortex and applied an ultracentrifugal and chromatographic prefractionation principle followed by two-dimensional gel electrophoresis (2-DE) and mass-spectrometrical analysis using high-throughput automated MALDI-TOF=TOF.. Nine Chr21Ps were identified: pyridoxal kinase; superoxide dismutase [Cu=Zn] 1; carbonyl reductase 1; ES1 protein homolog, mitochondrial [Precursor]; cystathionine-beta-synthetase; T-complex protein 1, theta subunit; cystatin B; 6-phosphofructokinase; glycinamide ribonucleotide synthetase.

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells
Article Snippet: The second dimension electrophoresis was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments, San Francisco, USA).

Article Title: Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach
Article Snippet: The IPG strips were equilibrated to reduce the disulfide linkages through the addition of 10 ml of equilibrating solution containing isopropanol and 2.6% tributyl phosphine (Fluka) and then were gently rocked for 25 min. Second-dimension electrophoresis was performed using 9-16% gradient gels and the Iso-DALT apparatus (Hoefer Scientific Instruments, San Francisco, CA), and was then stopped when the tracking dye reached the anode end of the gels.

Article Title: Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells.
Article Snippet: The second dimension electrophoresis was performed in an ISO-DALT apparatus (Hoefer Scientific Instruments, San Francisco, USA).

Article Title: Comparative proteomic analysis reveals differentially expressed proteins regulated by a potential tumor promoter, BRE, in human esophageal carcinoma cells.
Article Snippet: Esophageal tumorigenesis is a complex and cascading process, involving the interaction of many genes and proteins.. In this study, we have used the comparative proteomic approach to identify tumor-associated proteins and explore the carcinogenic mechanisms.. Two-dimensional electrophoresis (2-DE) and MALDI-TOF MS analysis of esophageal carcinoma and control cells revealed 10 proteins that were upregulated.

Article Title: Cyclin I and p53 are differentially expressed during the terminal differentiation of the postnatal mouse heart.
Article Snippet: In this study, we have used Ki-67 and MF20 mAb to determine how extensively cardiomyocytes proliferate in the postnatal mouse heart.. It was established that the cardiomyocytes divided rapidly in 2-day-old hearts.. However, at 13 days, the majority of cardiomyocytes had entered into terminal growth arrest and differentiation.



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Hoefer iso-dalt apparatus
Iso Dalt Apparatus, supplied by Hoefer, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iso-dalt+apparatus/iso+dalt+apparatus/pmc03033794-218-8-10
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